Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 14;12:7190. doi: 10.1038/s41467-021-27329-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Overview depicting the phenotypic characterization of the metabolic drug library. Selected graphics were modified from Servier Medical Art, licensed under a Creative Common Attribution 3.0 Generic License, http://smart.servier.com/. b Scatter dot plot showing the average top 25 effective compounds in the myeloid cell lines tested with each circle representing one cell line (n = 15 biologically independent cell lines in total) and bars labeled per metabolic process the corresponding compounds target. Graph shows mean ± SD. c Heatmap illustrating the functional grouping of myeloid cancer cells in five taxonomic groups based on metabolic vulnerability profiles. While each cell line had an overall distinct drug sensitivity profile, the activity of 18 compounds in particular significantly functionally stratified the myeloid cancer cells lines (an ordinary one-way ANOVA analysis *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; FDR of 10% was deemed significant *Padj ≤ 0.1; **Padj ≤ 0.05; ***Padj ≤ 0.01; ns not significant). d Measurement of oxygen consumption rate using Seahorse analyzer at basal level and after consecutive injections of oligomycin (1 μM), FCCP (1 μM), and antimycin A/rotenone (1 μM) (n = 8 technical replicates for each cell line n = 15). Error bars indicate mean ± SD of the data of cell lines falling in the same group. e XF PhenoGram profile of each of the myeloid leukemia cell lines is shown by plotting the basal OCR and ECAR providing a snap-shot of the bioenergetics profiles of the cell lines. Cell lines are colored based on which group they fall in as shown in c. Data presented as mean ± SD; n = 8 technical replicates).