Fig. 3. Metabolic coregulation revealed by genotype to phenotype associations.

a Summary of the most recurrent mutational events as well as FAB subtype annotation of the cell line panel included in the study. b Bubble plot describing the significant genotype to phenotype associations discerned with a two-sided equal variance t-test between mutant and wild-type cases. The size of each bubble corresponds to adjusted P value significance level with FDR of 10% (*Padj ≤ 0.1; **Padj < 0.05; ***Padj < 0.01). c Validation of selected phenotype to genotype associations (where data available) from the Beat AML study³³ (data retrieved from http://www.vizome.org/aml/). Box plots showing sensitivity of several metabolic modifiers that have significantly higher or lower sensitivity in FLT3, RAS, and TP53 mutant vs. wild-type AML patient samples. The number of independent samples for each comparison is indicated in the figure. Higher values, here, indicate lack of sensitivity and lower values indicate sensitivity. Error bars signify mean ± SD and the difference in response was assessed with either a two-tailed unpaired t-test or Mann–Whitney test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001).