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. 2021 Dec 14;11:23735. doi: 10.1038/s41598-021-02804-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sex determination of AVH-1 based on proteomics and number of nuclear DNA fragments aligning to the X chromosome and the autosomes. (A) Ion chromatograms of peptide SIRPPYPSY (AMELX, 540.2796 m/z) as in²²; peptide SM(ox)IRPPY (AMELY) has not been detected (or any other AMELY peptide), reflecting female status; (B) Dashed lines represent the expected ratios of X to (X + autosomal) fragments for a female and a male. Results were concordant for all fragments (blue dot) and for deaminated fragments (red dot). Vertical bars depict the 95% binomial confidence intervals.