Fig. 7. Knockdown of LUC7L2 increased autophagy level in NPC-radioresistant cells.
A Knockdown of LUC7L2 in CNE2IR increased LC3-II expression level after 24 h treatment of 50 μM CQ. Knockdown of LUC7L2 in Hone1IR slightly influenced the LC3-II level. β-actin served as a loading control. B CNE2IR and Hone1IR cells (shNEG and shLUC7L2) were treated with 50 μM CQ for 24 h, then stained for LC3 (green) and DAPI (blue). Bar: 50 μm. C Quantification of LC3 puncta based on the immunofluorescent images (n = 8).