Figure 3.

Direct sampling enables efficient AAV particle characterization and quantification by CD-MS

(A) Optimized sensitive approach for AAV particle characterization and quantification. Speed and required input amount are significantly reduced as no buffer exchange is needed. Here, the depicted workflow can be executed in ∼30 min per sample. (B) Illustrative 2D histogram of a dataset acquired in 20 min. For fast and easy quantification, all ions that fall in the margins of the drawn polygon are counted as either empty, partially full, or full AAV particles. (C) Corresponding mass histogram and bar plot depicting the most abundant masses for each species as well as their relative abundance.