Table 1.
Outline of the study.
| Day | Test | Treatment | Remark | |
|---|---|---|---|---|
| -7 | Pot jumping test (50-72) | saline ip. | Routine maintenance training baseline performance | |
| -6 | MWM-NE (50-60) | 5-CSRTT 1s (62-72) | saline ip. | |
| -5 | MWM-NE (62-72) | 5-CSRTT 1s (50-60) | saline ip. | |
| -4 | Cooperation task (in pairs) | saline ip. | ||
| -3 | Nose-poking with inactive lever (50-72) | saline ip. | Routine training | |
| -2 | Lever-press training (50-72) | saline ip. | New learning | |
| -1 | Lever-press training (50-72) | saline ip. | ||
| Randomization to treatment groups | ||||
| 1 | Motor activity (50-72) | CE-123/vehicle | ||
| 2 | CE-123/vehicle | |||
| 3 | Cooperation task (in pairs) | CE-123/vehicle | Standard assay conditions | |
| 4 | MWM-NW (50-5D) | 5-CSRTT 0.25s (5E-72) | CE-123/vehicle | Challenge conditions |
| 5 | MWM-NW (5E-72) | 5-CSRTT 0.25s (50-5D) | CE-123/vehicle | Challenge conditions |
| 6 | NP-LP (50-72) | CE-123/vehicle | Novel task | |
| 7 | CE-123/vehicle | |||
| 8 | CE-123/vehicle | |||
| 9 | NP-LP (50-72) | CE-123/vehicle | Novel task | |
| 10 | Cooperation task (in pairs) | CE-123/vehicle | Standard assay conditions | |
| 11 | MWM-center (50-5D) | 5-CSRTT 0.25s (5E-72) | CE-123/vehicle | Challenge conditions |
| 12 | MWM-center (5E-72) | 5-CSRTT 0.25s (50-5D) | CE-123/vehicle | Challenge conditions |
| 13 | Cooperation task (in pairs) | CE-123/vehicle | Standard assay conditions | |
| 14 | Pot jumping test (50-72) | CE-123/vehicle | Standard assay conditions | |
| 15 | Motor activity (50-72) | NP-LP (50-72) | CE-123/vehicle | Novel task |
| sacrifice (blood sampling, brain dissection) | ||||
Numbers in brackets show the hexadecimal identifiers of the animals, for MWM a position of the platform is indicated (NE, NW or center).