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. 2021 Dec 14;11:24003. doi: 10.1038/s41598-021-03165-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Supervised Orthogonal partial least squares discriminant (OPLS-DA) model with 13 variables selected from the 24 variables model with variable importance for projections (VIP) > 1: reductionist model. (A) Score plot generated from fertile (labeled as green triangles) and infertile couples (labeled as red dots). R² and Q² were 0.467 and 0.410, respectively. Accuracy (internal validation) = 0.838 and calculated on the test set = 0.705. (B) VIP (Variable Importance for the Projection) the plot summarized the influence of the 13 variables on the model. (C) Score plot reductionist model from the merged development and test sets (61 couples) (black stars). (D) Score plot reductionist model from the combined development and test sets. Scores are labeled according to their fertility status (green or red).