Skip to main content
. 2021 Dec 14;12:7268. doi: 10.1038/s41467-021-27197-5

Fig. 5. Homozygous expression of mutant IL7R leads to maximal IL-7R signaling hyperactivation and rapidly fatal leukemia.

Fig. 5

a Dot plots of CD19 by TCRβ to identify T and B cell lymphocytes in blood at 6 weeks of age in representative animals of indicated genotypes (left) and graphs summarizing data from all animals analyzed (right). Ctrls: n = 8; IL-7RmutHet: n = 10; IL-7RmutHom: n = 5. Each dot denotes an animal and mean value is shown. Numbers in dot plots indicate frequency in each quadrant. One-way ANOVA. b Kaplan–Meier leukemia-free survival curves of control (n = 40), IL-7RmutHet (n = 63), and IL-7RmutHom (n = 20) mutant animals. Log-rank (Mantel–Cox) test. All mice died with precursor B-ALL. c Immunophenotypic analysis of three representative BM IL-7RmutHom leukemia samples. Numbers inside dot plots indicate frequency in each quadrant or region. d Comparison of IL-7R “signaling strength” between IL-7RmutHet and IL-7RmutHom leukemia samples, as measured by the levels of differential gene expression over control samples. Each dot represents log2 fold change (FC) of an IL-7R signaling target gene (see Fig. 4b). Means are indicated. Unpaired two-tailed t-test. e Clonality pie charts based on IgH sequencing (see Fig. 2c for further details). Source data are provided as a Source Data file.