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. 2021 Nov 11;298(1):101406. doi: 10.1016/j.jbc.2021.101406

Figure 6.

Figure 6

Regulation of GM-CSF by PELP1.A and B, represent PELP1 overexpression by Western blot and quantitative RT–PCR in RAW 264.7 clones. C, GM-CSF expression in RAW 264.7-PELP1 overexpression clones by quantitative RT–PCR analysis. D and E, Western blots showing reduced expression of c-Rel when transfected with two different c-Rel siRNAs having different target sequences in RAW 264.7. E, also showed downregulation of PELP1 in c-Rel siRNA-transfected RAW 264.7 cells. F, ELISA analysis of GM-CSF expression regulated by PELP1 when transfected with c-Rel siRNA in RAW 264.7-PELP1 clones. G, Western blot and its quantification images representing PELP1 downregulation when transfected with c-Rel siRNA in RAW 264.7-PELP1 clones. H, inflammasome assay on RAW 264.7 PELP1 overexpression cells. Clones were treated with LPS (1 μg/ml for 4 h), and luminescence was measured after 60 min of incubation with Caspase-Glo-1 reagent. GM-CSF, granulocyte–macrophage colony-stimulating factor; LPS, lipopolysaccharide; PELP1, proline, glutamic acid, and leucine-rich protein 1.