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. 2021 Nov 11;298(1):101406. doi: 10.1016/j.jbc.2021.101406

Figure 9.

Figure 9

Feedback regulation of PELP1 expression in cancer.A and B, Western blot and quantitative RT–PCR analysis of PELP1 expression in HC11 cells incubated with CM collected from RAW 264.7-PELP1 clones for 6 h. β-Actin was used as control. C, Western blot analysis of PELP1 expression in HC11 cells when incubated with CM/GM-CSF–depleted CM collected from RAW 264.7-PELP1 clones for 6 and 12 h time intervals. D, Western blot analysis of various cytokine-mediated signaling pathway proteins along with PELP1 in HC11 cells incubated with CM collected from RAW 264.7-PELP1 clones. E, Western blot analysis of HC11 cells pretreated with S31-201 inhibitor (86 μM) followed by CM treatment collected from RAW 264.7-PELP1 clones. Actin and total STAT3 as loading controls. CM, conditioned media; GM-CSF, granulocyte–macrophage colony-stimulating factor; PELP1, proline, glutamic acid, and leucine-rich protein 1.