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. 2001 Mar;21(5):1710–1718. doi: 10.1128/MCB.21.5.1710-1718.2001

FIG. 5.

FIG. 5

A large region of chromosome VII flanked by two homologous tRNA genes is frequently deleted. (A) Marker strains with (lane 1) or without (lane 2) the control chromosome; 13 strains identified as large internal deletions from rad51 CDC5 (lanes 3 to 15) or rad51 cdc5-ad (lanes 16 to 28) were run on a CHEF gel, blotted, and probed for the test chromosome. (B) DNA from the starting strain or a strain containing a large internal deletion (as in panel A, lane 3) was fluorescently labeled and hybridized to a DNA array as described previously (9). Hybridization ratios are shown for genes on the left arm of chromosome VII. Arrows designate leucine tRNA genes flanking the deletion. (C) Oligonucleotides corresponding to the outside of the deletion were used in a PCR on the strains shown in panel A. M, marker. (D) The putative SSA intermediate between the centromere proximal [TL (CAA) G2] and distal [TL (CAA) G1] tRNA genes flanking the internal deletions mapped in panels A to C. The oligonucleotides used in panel C are indicated as arrows.