TABLE 1.
Genotype | Frequency (%) of chromosome loss (mean ± SD)
|
|
---|---|---|
Spontaneousb | X-ray inducedc | |
rad52 CDC5 CKB2d | 1.2 ± 0.21 | 16.3 ± 4.2 |
rad52 cdc5-ad CKB2d | 0.26 ± 0.07 | 1.7 ± 1.2 |
rad52 CDC5 ckb2 | 0.29 ± 0.02 | 1.9 ± 1.4 |
rad51 CDC5 | 0.30 ± 0.071 | 13.8 ± 0.41 |
rad51 cdc5-ad | 0.073 ± 0.21 | 5.5 ± 1.1 |
Strains used harbored an extra copy of chromosome VII, which contains the CYH2 and ADE3 genes.
Strains were plated onto medium containing cycloheximide, which selects for cells that have lost the CYH2 gene on the extra chromosome (see also Fig. 3).
Cells were plated on nonselective medium and immediately X-irradiated with 2 (rad52 strains) or 3 (rad51 strains) kilorads.
This strain is pictured in Fig. 2.