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. 2001 Mar;21(5):1710–1718. doi: 10.1128/MCB.21.5.1710-1718.2001

TABLE 1.

Loss of chromosome VII in haploid strainsa

Genotype Frequency (%) of chromosome loss (mean ± SD)
Spontaneousb X-ray inducedc
rad52 CDC5 CKB2d 1.2 ± 0.21 16.3 ± 4.2
rad52 cdc5-ad CKB2d 0.26 ± 0.07 1.7 ± 1.2
rad52 CDC5 ckb2 0.29 ± 0.02 1.9 ± 1.4
rad51 CDC5 0.30 ± 0.071 13.8 ± 0.41
rad51 cdc5-ad 0.073 ± 0.21 5.5 ± 1.1
a

Strains used harbored an extra copy of chromosome VII, which contains the CYH2 and ADE3 genes. 

b

Strains were plated onto medium containing cycloheximide, which selects for cells that have lost the CYH2 gene on the extra chromosome (see also Fig. 3). 

c

Cells were plated on nonselective medium and immediately X-irradiated with 2 (rad52 strains) or 3 (rad51 strains) kilorads. 

d

This strain is pictured in Fig. 2