FIG. 4.

Neither inhibitors of transcription nor translation blocks the assembly of XL53BP1. XTC-2 cells were irradiated with 5 Gy of γ rays in the presence of 50 μg of cycloheximide (Cyh) per ml (C) or 25 μg of actinomycin D (Act) per ml (D). (A) Nonirradiated control. (B) Cells irradiated without the inhibitors. (E) the amount of XL53BP1 did not increase after γ-ray irradiation. XTC-2 cells were exposed to 5 or 15 Gy of γ rays, and at 2 h after irradiation, cell extracts were prepared for immunoblotting with anti-XL53BP1 antibody. Each slot was loaded with the same amount of proteins.