Figure 2.

BCG-induced T cell responses and gene expression changes are enhanced upon BCG vaccination. a Paired point graphs depicting magnitude of IFNγ responses pre- and post-vaccination, against BCG and PHA as SFC per 10⁶ cultured PBMC as determined by Fluorospot. Each point and symbol represent one participant (n=12), median ± interquartile range is shown. (Wilcoxon matched pair signed rank test). b PCA depicting the variation in global gene expression as a result of stimulation condition and vaccination. Pre-vaccination (open circles) and post vaccination (closed circles), unstimulated samples (blue, n=7) and BCG stimulated samples (gold, n=6). c Volcano plot depicting differentially expressed genes after BCG stimulation as compared to unstimulated samples, pre- and post-vaccination. Red indicates upregulated genes (adjusted p value < 0.05 and log₂ fold change > 1) and blue indicates downregulated genes (adjusted p value < 0.05 and log₂ fold change < -1: Benjamini Hochberg corrected DESeq2 Wald test).