Figure 7.

BCG and MTB300 stimulation elicit specific T cell responses. a Number of TCR clonotypes covering 80% of the productive repertoire across stimulation conditions and pre- and post-vaccination. Colour, yellow and green, represents pre- and post-vaccination, respectively. Each symbol represents one donor (n=8). Replicate samples are indicated by the intensity of the colour (lighter and darker shade). b TCR-sequencing was performed on CD4⁺ T cell samples obtained from BCG naïve individuals (n=8), stimulated with the BCG epitope pool, MTB300 peptide pool, or the tetanus peptide pool. Only those clonotypes that expanded upon stimulation compared to ex-vivo CD4 samples across both replicates were retained within each stimulation condition. The resulting clonotypes in the three stimulation conditions were overlapped to obtain clonotypes that expanded upon BCG stimulation only, MTB300 stimulation only, and clonotypes expanded in both BCG and MTB300 stimulation. Any clonotype expanded upon tetanus stimulation was excluded. This process was repeated for samples obtained post-vaccination. c Bar graphs showing the number of expanded clonotypes, and the total templates for these clonotypes, across different conditions, pre- and post-vaccination. Blue indicates clonotypes unique to post-vaccination, green indicates clonotypes unique to pre-vaccination, and grey indicates clonotypes that expanded in both pre- and post-vaccination. d Quantification of total templates expanded in pre- and post-vaccination for individual donors in each condition. Empty and filled circles represent pre- and post-vaccination, respectively.