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. 2021 Dec 1;12:775659. doi: 10.3389/fimmu.2021.775659

Figure 1.

Figure 1

Schematic of P. falciparum CSP and region-specific antigens used in this study. (A) Native P. falciparum CSP (3D7) is 397 amino acids in length and can be divided into the N-terminal region that contains Region I (RI), the central-repeat region largely comprised of NANP tandem repeats, and the C-terminal region that includes Region III (RIII) and the thrombospondin type 1 repeat (TSR) domain. (B) In this study, we used two near full-length CSP antigens that lacked the signal peptide and GPI sequences from Gennova (expressed in E. coli) and Sanaria (expressed in P. pastoris) in human and rabbit studies, respectively. The Gennova CSP contained the major NANP motif repeated 37 times, and the Sanaria CSP began at amino acid 50 and contained the major NANP motif repeated 22 times and the minor NVDP motif repeated 4 times. (C) We also used region-specific antigen constructs including a peptide representing the N-terminal region between amino acids 19-104 (NT), a peptide representing the central-repeat region comprised of the NANP motif repeated 15 times (NANP), recombinant protein representing the C-terminal region between amino acids 273-383 (CT) and a truncated form of CSP generated by the N-terminal and C-terminal regions fused together via a GGGS short linker (N+C). See Table 1 for full details. Created using BioRender.com.