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. 2021 Dec 1;12:775659. doi: 10.3389/fimmu.2021.775659

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Copyright © 2021 Kurtovic, Drew, Dent, Kazura and Beeson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rabbit IgG to the non-repeat regions of CSP can fix complement. Rabbit IgG were generated against (A) truncted CSP that lacked the central-repeat region (N+C) and the (B) C-terminal (CT) and (C) N-terminal (NT) regions of CSP. Rabbit IgG were tested for IgG at 0.001-10 μg/ml (left) and C1q-fixation at 0.1-1000 μg/ml (right) against full-length CSP, NT, NANP and CT anitgens [and N+C for panel (A)]; x-axis was presented on a log10 scale. Results were corrected for background reactivity using no-IgG control wells and the mean and range of two independent experiments is shown.