Table 1.
Summary of P. falciparum antigens used in the study based on the 3D7 strain.
| Antigen | Origin | Description/Sequence | Ref. |
|---|---|---|---|
| CSP (Gennova) | E. coli | Begins and ends at amino acids 19 and 383 of CSP, respectively, and contains the major NANP motif repeated 37 times | (36) |
| CSP (Sanaria) | P. pastoris | Begins and ends at amino acids 50 and 384 of CSP, respectively, and contains the major NANP motif repeated 22 times and the minor NVDP motif repeated 4 times | (16, 37) |
| NT (Life Tein) | Peptide | Amino acids 19-104 of CSP whereby the Cys25 was modified to Ser25. Sequence: LFQEYQSYGSSSNTRVLNELNYDNAGTNLYNELEMNYYGKQENWYSLKKNSRSLGENDDGNNEDNEKLRKPKHKKLKQPADGNPDP | (38) |
| NANP (Life Tein) | Peptide | Major NANP motif repeated 15 times. Sequence: NANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANP | (23) |
| CT (in-house) | HEK293 | Amino acids 237-383 of CSP. Sequence: NKNNQGNGQGHNMPNDPNRNVDENANANSAVKNNNNEEPSDKHIKEYLNKIQNSLSTEWSPCSVTCGNGIQVRIKPGSANKPK DELDYANDIEKKICKMEKCSSVFNVVNSGS |
(23) |
| N+C (in-house) | HEK293 | Amino acids 26-104 and 273-383 of CSP (as above) fused with GGGS linker | (38) |