Figure 6.

MIAT knockdown significantly ameliorated cardiac pyroptosis in vivo

(A) Representative immunohistochemistry heart images of CASP1 and IL-1β in mice with indicated treatments.

(B–E) The relative gene expression levels of MIAT (B), miR-214-3p (C), CASP1 (D), and IL-1β (E) in control, DM, DM + LV-siMIAT, and DM + CASP1 I groups.

(F) The relative protein expression levels of CASP1, IL-1β, IL-18, and GSDMD in control, DM, DM + LV-siMIAT, and DM + CASP1 I groups were detected by western blotting. Densitometry analysis was performed and demonstrated as fold change, normalized to GAPDH.

(G) is Densitometry analysis was performed and demonstrated as fold change, normalized to GAPDH.

(H) I_Ca,L was detected by patch clamping The baseline and peak fluorescence intensity were demonstrated as line charts. Scale bar: 20 μm. n = 5 for each experiment. Treatment using verapamil (20umol/L) to block calcium channel was used as a positive control ∗∗p < 0.01, ∗∗∗p < 0.001 versus the control group. ^#p < 0.05, ^##p < 0.01 versus DM, ^###p < 0.001 versus DM.