. 2021 Nov 26;24(12):103518. doi: 10.1016/j.isci.2021.103518

Table 4.

PCR primer sequence

Primer	RNA sequence (5′-3′)		Product length (bp)
U6	Forward	CTCGCTTCGGCAGCACATATACT	95
U6	Reverse	ACGCTTCACGAATTTGCGTGTC	95
MIAT	Forward	TGGAACAAGTCACGCTCGATT	150
MIAT	Reverse	GGTATCCCAAGGAATGAAGTCTGT	150
CASP1	Forward	ACACGTCTTGCCCTCATTATCT	365
CASP1	Reverse	ATAACCTTGGGCTTGTCTTTCA	365
IL-1β	Forward	CCCTGCAGCTGGAGAGTGTGG	153
IL-1β	Reverse	TGTGCTCTGCTTGAGAGGTGCT	153
GAPDH	Forward	ATCACTGCCACCCAGAAGAC	211
GAPDH	Reverse	TTTCTAGACGGCAGGTCAGG	211
miR-214-3p	miRNA quantification: Bulge-loop^TM miRNA qRT-PCR primer sets (one RT primer and a pair of qPCR primer for each set) specific for miR-214-3p are designed by RiboBio (Guangzhou, China) (Bulge-Loop^TM miR-214-3p Reverse Primer: No. ssD089261711, Bulge-Loop^TM miR-214-3p Forward Primer: No. ssD809230935, Bulge-Loop^TM miR-214-3p RT Primer: No. ssD809230243)