Identification of host cell S100A6 and ANXA6's co-localization with TgSAG1 when T. gondii is attaching to host cells
Co-localization of TgSAG1 with its interactive proteins in RH-WT infected HFF cells were visualized by IFA. The primary antibodies, rabbit S100A6, ANXA6, MYH9, CKAP4, and HA-tag antibody, followed by the second antibody Donkey anti-rabbit IgG conjugated with Alexa Fluor 594 were used to detect S100A6, ANXA6, MYH9, and CKAP4, whereas mouse anti DPP4, ITGB1, SAG1 antibody followed by Goat anti-mouse IgG conjugated with Alexa Fluor 488 were used to detect DPP1, ITGB1, and SAG1(scale bars, 10 μm).
See also Figure S2.