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. 2021 Nov 26;24(12):103514. doi: 10.1016/j.isci.2021.103514

Figure 7.

Figure 7

TgSAG1 promoted TNF-α secretion in THP-1 cells

(A–C) Transcriptional levels of IL-12p70, IFN-γ, TNF-α in RH-WT or RH-Δsag1 infected THP-1 cells were detected by qRT-PCR.

(D) The secretion of TNF-α in RH-WT or RH-Δsag1 infected THP-1 cells were detected by ELISA. Data were presented as pg/mL.

(E) Verification of TgSAG1 overexpression in RAW264.7 cells by WB.

(F and G) the transcription and secretion of TNF-α in TgSAG1 transfected RAW264.7 cells were detected by qRT-PCR and ELISA, respectively. Data were presented as means ± SD combined from three independent experiments and statistical significance were analyzed by t test (∗∗p < 0.05).