Antibodies |
|
Anti-Toxoplasma gondii antibody |
Abcam |
Cat#ab8313 |
RRID: AB_306466
|
Anti-DPP4 |
Abcam |
Cat#ab114033 |
RRID: AB_10901695
|
Anti-ITGβ1 |
Abcam |
Cat#ab30394 |
RRID: AB_775726
|
Anti-MYH9 |
Abcam |
Cat#ab24762 |
RRID: AB_2282292
|
Anti-CKAP4 |
Thermo Fisher Scientific |
Cat#PA5-100059 |
RRID: AB_2815589
|
Anti-ANXA6 |
Thermo Fisher Scientific |
Cat#720160 |
RRID: AB_2609027
|
Anti-vVimentin |
Cell Signaling Technology |
Cat#D21H3 |
RRID: AB_10695459
|
Anti-HA tag |
Cell Signaling Technology |
Cat#3724S |
RRID: AB_1549585
|
Anti-DDDK |
Sigma Aldrich |
Cat#F1804 |
RRID: AB_262044
|
Anti-Actin |
Abcam |
Cat#ab179467 |
RRID: AB_2737344
|
Anti-S100A6 |
Thermo Fisher Scientific |
Cat#PA5-97153 |
RRID: AB_2808955
|
Anti-NF-κB |
Cell Signaling Technology |
Cat#8242 |
RRID: AB_10859369
|
Anti-p NF-κB |
Cell Signaling Technology |
Cat#3033S |
RRID: AB_331284
|
Anti-PKCθ |
Abcam |
Cat#ab76197 |
RRID: AB_1310589
|
Goat anti-mouse IgG (H+L) secondary antibody, HRP |
Abclonal |
Cat#AS003 |
RRID: AB_2769851
|
Goat anti-rabbit IgG (H+L) secondary antibody, HRP |
Abclonal |
Cat#AS014 |
RRID: AB_2769854
|
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat#A11001 |
RRID: AB_2534069
|
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher Scientific |
Cat#A11032 |
RRID: AB_2534091
|
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat#A32731 |
RRID: AB_2633280
|
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
|
Thermo Fisher Scientific
|
Cat#A32740 |
RRID: AB_2762824
|
|
Chemicals, peptides, and recombinant proteins |
|
Sotrastaurin |
Selleck |
S2791 |
Lipofectamine™ 3000 Transfection Reagent |
Thermo Fisher Scientific |
L3000 |
D-Biotin |
Thermo Fisher Scientific |
B1595 |
eBioscience™ Phalloidin eFluor™ 570 |
Thermo Fisher Scientific |
41-6559-05 |
|
Critical commercial assays |
|
Human TNF-α kit |
MULTI SCIENCES |
EK182 |
Mouse TNF-α kit |
MULTI SCIENCES |
EK282/3 |
|
Experimental models: Cell lines |
|
THP-1 cells |
ATCC |
TIB-202 |
COS-7 cells |
ATCC |
CRL-1651 |
HFF cells |
ATCC |
SCRC-1041 |
|
Experimental models: Organisms/strains |
|
RH-SAG1-TurboID-HA |
This paper |
N/A |
RH-Δsag1
|
This paper |
N/A |
|
Oligonucleotides |
|
Primer for qPCR was shown in Table S1
|
This paper |
N/A |
|
Software and algorithms |
|
Prism |
Graph Pad |
https://www.graphpad.com/scientific-software/prism/ |
ImageJ |
Java |
https://imagej.en.softonic.com/ |