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. 2021 Nov 26;24(12):103514. doi: 10.1016/j.isci.2021.103514
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-Toxoplasma gondii antibody Abcam Cat#ab8313
RRID: AB_306466
Anti-DPP4 Abcam Cat#ab114033
RRID: AB_10901695
Anti-ITGβ1 Abcam Cat#ab30394
RRID: AB_775726
Anti-MYH9 Abcam Cat#ab24762
RRID: AB_2282292
Anti-CKAP4 Thermo Fisher Scientific Cat#PA5-100059
RRID: AB_2815589
Anti-ANXA6 Thermo Fisher Scientific Cat#720160
RRID: AB_2609027
Anti-vVimentin Cell Signaling Technology Cat#D21H3
RRID: AB_10695459
Anti-HA tag Cell Signaling Technology Cat#3724S
RRID: AB_1549585
Anti-DDDK Sigma Aldrich Cat#F1804
RRID: AB_262044
Anti-Actin Abcam Cat#ab179467
RRID: AB_2737344
Anti-S100A6 Thermo Fisher Scientific Cat#PA5-97153
RRID: AB_2808955
Anti-NF-κB Cell Signaling Technology Cat#8242
RRID: AB_10859369
Anti-p NF-κB Cell Signaling Technology Cat#3033S
RRID: AB_331284
Anti-PKCθ Abcam Cat#ab76197
RRID: AB_1310589
Goat anti-mouse IgG (H+L) secondary antibody, HRP Abclonal Cat#AS003
RRID: AB_2769851
Goat anti-rabbit IgG (H+L) secondary antibody, HRP Abclonal Cat#AS014
RRID: AB_2769854
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat#A11001
RRID: AB_2534069
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 Thermo Fisher Scientific Cat#A11032
RRID: AB_2534091
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat#A32731
RRID: AB_2633280
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
Thermo Fisher Scientific
Cat#A32740
RRID: AB_2762824

Chemicals, peptides, and recombinant proteins

Sotrastaurin Selleck S2791
Lipofectamine™ 3000 Transfection Reagent Thermo Fisher Scientific L3000
D-Biotin Thermo Fisher Scientific B1595
eBioscience™ Phalloidin eFluor™ 570 Thermo Fisher Scientific 41-6559-05

Critical commercial assays

Human TNF-α kit MULTI SCIENCES EK182
Mouse TNF-α kit MULTI SCIENCES EK282/3

Experimental models: Cell lines

THP-1 cells ATCC TIB-202
COS-7 cells ATCC CRL-1651
HFF cells ATCC SCRC-1041

Experimental models: Organisms/strains

RH-SAG1-TurboID-HA This paper N/A
RH-Δsag1 This paper N/A

Oligonucleotides

Primer for qPCR was shown in Table S1 This paper N/A

Software and algorithms

Prism Graph Pad https://www.graphpad.com/scientific-software/prism/
ImageJ Java https://imagej.en.softonic.com/