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. 2021 Nov 16;297(6):101420. doi: 10.1016/j.jbc.2021.101420

Figure 5.

Figure 5

The enhanced ADCC activities of HER2/PD-L1 BsAb.A and B, HER2 and PD-L1 expression levels on HCC1954 and NCI-N87 cells were measured by flow cytometry. C and D, ADCC activities of HER2/PD-L1 BsAb and trastuzumab to HCC1954 and NCI-N87 cells using human PBMCs as effector cells. Tumor cells were cocultured with human PBMCs (1:20) in the presence of HER2/PD-L1 BsAb, trastuzumab, or isotype for 18 h. Supernatants were harvested to measure the LDH release by ELISA. E, the production of IFN-γ in tumor cells and human PBMC coculture was measured by ELISA. F, IFN-γ-induced upregulation of PD-L1 expression on NCI-N87 cells as determined by flow cytometry.∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 as indicated by Student’s t test. Data were represented as mean ± STE.