Figure 4.
P2X7-dependent cross-dressing induces the proliferation of CD8+ OT-1 lymphocytes
In a 1:1 ratio 1 x 105 CT Violet OT-1 lymphocytes were co-cultivated with allogeneic BMDC-BALB/c. The proliferation round of OT-1 lymphocytes was determined by the decrease in CT Violet fluorescence and counting peaks to the left. Representative histograms from 3 to 6 independent experiments.
(A) Proliferation rounds of OT-1 lymphocytes incubated with BMDC-BALB/c previously challenged with E-ACs (blue) and E-ACs-P2X7 (red) both loaded with SIINFEKL.
(B) The percentage of CD8+ OT-1 cells that completed a proliferation cycle (1–6) is shown for each treatment.
(C) The % CD25 + CD8+ OT-1 lymphocytes after stimulation. OT-1 CD8+ lymphocytes were incubated for 24 h with BMDC-BALB/c challenged with E-ACs (blue) and E-ACs-P2X7 (red) both SIINFEKL-loaded. The CD25 + CD8+ OT-1 population of each condition was normalized against untreated OT-1 splenocytes. Data are represented as mean ± SEM. Results were analyzed using non-parametric Mann-Whitney test, ∗ p < 0.05