REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
P2X7 Polyclonal Antibody	Thermo Fisher Scientific	CAT#PA5-28020; RRID: AB_2545496
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP	Thermo Fisher Scientific	CAT#A16110; RRID: AB_2534782
CD11c Monoclonal Antibody (N418), PE	Thermo Fisher Scientific	CAT#12-0114-82; RRID: AB_465552
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC	Thermo Fisher Scientific	CAT#11-5321-82; RRID: AB_465232
CD16/CD32 Monoclonal Antibody (93)	Thermo Fisher Scientific	CAT#14-0161-82; RRID: AB_467133
FITC anti-mouse CD11c Antibody	Biolegend	CAT#117306; RRID: AB_313775
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (25-D1.16), APC	Thermo Fisher Scientific	CAT# 17-5743-82; RRID: AB_1311286
PE Rat Anti-Mouse CD25	BD Bioscience	CAT# 561065; RRID: AB_395101
Bacterial and virus strains
pcDNA3-OVA plasmid	Diebold SS et al., 2001	RRID: Addgene_64599
P2X7-pIRES2/eGFP plasmid	Genscript Biotech	N/A
P2X7-pcDNA3.1 Hygro(+) plasmid	Genscript Biotech	N/A
Biological samples
Bone marrow-derived dendritic cells (BMDCs) from BALB/CJ, C57BL/6 and P2X7KO mice	Eduardo Morales Santos Facility of Universidad de Santiago de Chile	N/A
Chemicals, peptides, and recombinant proteins
Recombinant Mouse GM-CSF	Biolegend	CAT# 576304; Accession # NM_009969
Mouse IL-4 Recombinant Protein	Thermo Fisher Scientific	CAT#RMIL4I; GenBank Accession # P07750
Lipofectamine™ 2000 Transfection Reagent	Thermo Fisher Scientific	CAT# 11668019
Selection antibiotic G418	Merck	CAT#345810; CAS 108321-42-2
Hygromycin B (50 mg/ml)	Thermo Fisher Scientific	CAT# 10687010
CellTracker™ Red CMTPX Dye	Thermo Fisher Scientific	CAT# C34552
CellTracker™ Orange CMRA Dye	Thermo Fisher Scientific	CAT# C34551
CellMask™ Deep Red Plasma membrane Stain	Thermo Fisher Scientific	CAT# C10046
Ovalbumin (257-264) chicken ≥97%	Sigma-Aldrich	N/A
7-AAD Viability Staining Solution	Thermo Fisher Scientific	CAT#00-6993-50
Critical commercial assays
Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and Propidium Iodide (PI)	Thermo Fisher Scientific	CAT# V13241
Experimental models: cell lines
Mutu1940	Faculty of Chemical Sciences, National University of Córdoba, Argentina. Dr. Gabriel Morón	N/A
EL-4	ATCC	CAT#TIB-39
HEK293	ATCC	CAT#CRL-1573
Experimental models: Organisms/strains
Mouse: BALB/cJ	The Jackson Laboratory	CAT# 000651
Mouse: C57BL/6J	The Jackson Laboratory	CAT# 000664
Mouse: B6.129P2-P2RX7tm1Gab (P2X7 KO)	The Jackson Laboratory	CAT# 005576
Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-1)	The Jackson Laboratory	CAT#003831
Software and algorithms
GraphPad Prism version 8.01	GraphPad Software, Inc.	https://www.graphpad.com/scientific-software/prism/
FlowJo 10.4	Becton Dickinson	https://www.flowjo.com/solutions/flowjo/downloads/previous-versions
BD Accuri™ C6 plus	Becton Dickinson	https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-accuri-c6-plus
ZEN 2.6 blue edition	Carl Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html