Figure 5.

In vivo growth inhibition effect on HCC cells by 5-FU with or without CD44-Apt1conjugation. Subcutaneous HCC xenograft tumors were developed using Hep3B vector-control, CD44E-OE, or CD44s-OE cells. When xenograft reached size of about 100mm³, 250 pmol or 1nmol of CD44-Apt1-5FU (orange, square), or equivalent dosage of 5-FU (blue, circle) was intravenously injected for 8 consecutive days into tumor-bearing mice. Green arrows indicated the day of injection. The tumors were allowed to grow for 2 to 6 days before sacrifice. Tumor size and body weight were measured every two or every other day and compared between the two treatment groups using 2-way ANOVA test (mean ± SD). Tumors were weighted at the end point and compared between the two treatment groups using unpaired t-test. *p <0.05, **p <0.01, ***p<0.001, **** p<0.0001.