8% PAA gel

Reagent	Final concentration	Amount
30% solution Acrylamide to Bisacrylamide	8%	10.4 mL
urea	7M	16.8 g
5× TBE	1×	8 mL
100mg/mL Ammonium persulfate	0.5 mg/mL	0.2 mL
TEMED	0.1%	0.04 mL
Total	n/a	40 mL

Before adding ammonium persulfate and TEMED, the solution may be stored for some days in the dark, but we advise to use it immediately to pour the gel.