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. 2021 Dec 11;2(4):101001. doi: 10.1016/j.xpro.2021.101001
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit anti-IMPA1 (use 1:10000 for western blot) Abcam ab184165
Mouse anti-HDAC2 (use 1:1000 for western blot) Abcam ab12169

Chemicals,peptides, andrecombinantproteins

Recombinant Ago2 Active Motif 31486
Rat tail collagen Homemade n/a
PBS 0.0095M(PO4) w/o Ca and Mg Lonza BE17-516F
Protease Inhibitor Cocktail Merck P8340
RNasin Plus Ribonuclease Inhibitor Promega N2611
RNAseZap Merck R2020
Calf Intestinal Phosphatase (CIP) New England Biolabs M0290S
Sodium Acetate buffer solution Merck S7899
Linear acrylamide Thermo Fisher Scientific AM9520
Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Fisher Scientific 15875408
Ethanol Merck 32221
γ-32P ATP PerkinElmer BLU002A100UC
T4 Polynucleotide Kinase Thermo Fisher Scientific EK0031
0.5M EDTA pH 8 Merck E7889
TRIzol Reagent Thermo Fisher Scientific 15596018
RNA Loading Dye, (2×) New England Biolabs B0363S
ProtoGel (30% Solution at 37.5:1 Ratio) GENEFLOW EC-890
Ammonium persulfate Merck A3678
TEMED VWR 443083g
Trizma base Merck T1503
Boric acid Meck B6768
Urea Merck U5578
HEPES Buffer, 1M Solution Fisher Scientific 83264
Potassium hydroxide Merck 60377
Potassium acetate Merck 60035
Magnesium Acetate Merck M5661
DL-Dithiothreitol Merck D9779
Yeast tRNA (10 mg/mL) Thermo Fisher Scientific AM7119

Experimentalmodels: Celllines

rat sympathetic neuron extracts This paper N/A

Experimentalmodels: Organisms/strains

Wild-type P0/P1 Wistar rats, either sex N/A N/A

Oligonucleotides

CTGACACATTAGAAGCAGGAATTGCAGCTTCA
AGCACTCAGTGTGAGGTGTCGATTGGAGATCG
CCTACTGTCTGACTGGCTCTGCACTAGGTGCT
GTATTACAGTGATTAACCTGTCTC
Sigma-Genosys N/A

Other

NE-PER™ Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific 78833
Decade Markers System Thermo Fisher Scientific AM7778
mirVana™ miRNA Probe Construction Kit Thermo Fisher Scientific AM1550