RNAse-free water	75 μL
2.0 M sodium acetate pH 4.3	15 μL
Phenol/chloroform 25:24:1	150 μL

Mix well by vigorous shaking of the tube by hand, then spin at 17000×g at 22°C–25°C for 5 min in a microcentrifuge. Transfer the aqueous (top) phase to a clean 1.5 mL tube.