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Performing spot detection and tracking binaries
(A) Progression of a deconvolved z stack to a maximum intensity projection. The maximum intensity projected image is then used for spot detection.
(B) Spot detection setup.
(C) Track Binaries setup.
(D) Frequency distribution setup in Prism 10.
(E) Example of EGFP-EPS8 puncta intensity histogram. n = number of puncta from a single representative cell (reprinted with permission from Gaeta et al., 2021).
