Imaging medium

Reagent	Final concentration	Amount
FluoroBrite DMEM	n/a	500 mL
Fetal bovine serum	10%	50 mL
L-glutamine	1%	5.5 mL
Total	n/a	555.5 mL

Store complete FluoroBrite DMEM at 4°C for 4–6 weeks. Prewarm media to 37°C before use.