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| Reagent | Final concentration | Amount |
|---|---|---|
| Puromycin dihydrochloride | 10 mg/mL | 25 mg |
| MiliQ H20 | n/a | 2.5mL |
| Total | n/a | 2.5 mL |
Filter sterilize using a 10 mL syringe equipped with 0.22 μm filter, aliquot and store at −20°C for up to two years. Keep working stock at 4°C for up to one year.
