| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-CD31 (use at 1:200) | BD Pharmingen | Cat#553370 |
| Rabbit anti-VE-Cadherin (use at 1:500) | Abcam | Cat#ab33168 |
| Goat anti-Iba1 (use at 1:1000) | Abcam | Cat#ab107159 |
| Goat anti-PDGRβ (use at 1:200) | R&D systems | Cat#AF1042 |
| Guinea pig anti-NeuN (use at 1:1500) | Millipore Sigma | Cat#ABN90 |
| Guinea pig anti-GFAP (use at 1:1000) | Synaptic Systems | Cat#173004 |
| Mouse anti-αSMA (use at 1:200) | Sigma-Aldrich | Cat#A2547 |
| Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (use at 1:300) | Invitrogen | Cat#A-21208 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (use at 1:300) | Invitrogen | Cat#A-10042 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (use at 1:300) | Invitrogen | Cat#A-31573 |
| Donkey anti-goat IgG (H+L) cross-absorbed secondary antibody, Alexa Fluor 568 (use at 1:300) | Invitrogen | Cat#A-11057 |
| Goat anti-guinea pig IgG (H+L) Highly cross-absorbed secondary antibody, Alexa Fluor 488 (use at 1:300) | Invitrogen | Cat#A-11073 |
| Goat anti-guinea pig IgG (H+L) Highly cross-absorbed secondary antibody, Alexa Fluor 647 (use at 1:300) | Invitrogen | Cat#A-21450 |
| Donkey anti-mouse IgG (H+L) cross-absorbed secondary antibody, Alexa Fluor 568 (use at 1:300) | Invitrogen | Cat#A10037 |
| Alexa Fluor™ 488 Phalloidin (use at 1:40) | Invitrogen | Cat#A12379 |
| Chemicals, peptides, and recombinant proteins | ||
| EGMTM-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM | Lonza | Cat#CC-3202 |
| Penicillin-Streptomycin (10,000 U/mL) | Thermo Fisher Scientific | Cat#15140122 |
| HBSS without calcium/magnesium | Gibco | Cat#14170-120 |
| HBSS with calcium/magnesium | Gibco | Cat#14025-092 |
| Attachment factor 1× | Gibco | Cat#S-006-100 |
| Albumin, Bovine (BSA) | VWR | Cat#0332-100G |
| CD31 MicroBeads, Mouse | Miltenyi Biotec | Cat#130-097-418 |
| Myelin Removal Beads II, human, mouse, rat | Miltenyi Biotec | Cat#130-096-733 |
| CD45 MicroBeads, Mouse | Miltenyi Biotec | Cat#130-052-301 |
| Sterile 1× PBS | Wisent BioProducts | Cat#311-011-CL |
| TrypLE™ Express Enzyme (1×), no phenol red | Thermo Fisher Scientific | Cat#12604013 |
| Alconox detergent | VWR | Cat#21835-032 |
| Virkon® S Disinfectant and Virucide | Fisher Scientific | Cat#NC9549979 |
| CellEvent™ Caspase-3/7 Green Detection | Thermo Fisher Scientific | Cat#C10423 |
| Staurosporine | Thermo Fisher Scientific | Cat# BP2541100 |
| Hoechst 33342, Trihydrochloride, Trihydrate-10 mg/mL Solution in Water | Invitrogen | Cat#H3570 |
| Paraformaldehyde 20% Solution, EM grade | Electron Microscopy Sciences | Cat#15713-S |
| Fluoromount-G™ | Electron Microscopy Sciences | Cat#17984-25 |
| Triton-X-100 | VWR | Cat#0694-1L |
| Fish gelatin blocking buffer, 10% | VWR | Cat#M319-500mL |
| Donkey serum, sterile filtered | Wisent Bioproducts | Cat#035-150 |
| Dimethyl sulfoxide,DMSO | sigma-Aldrich | Cat#D8418-100mL |
| DAPI | Invitrogen | Cat#D1306 |
| Matrigel® Matrix Basement Membrane, Growth Factor Reduced | Fisher Scientific | Cat#354230 |
| Sodium chloride (NaCl) | VWR | Cat#0241-5KG |
| Sodium phosphate dibasic anhydrous (Na2HPO4) | VWR | Cat#0404-1KG |
| Sodium phosphate monobasic (NaH2PO4H2O) | VWR | Cat#BDH9298.2.5 |
| β-mercaptoethanol, Molecular biology grade | Merck Millipore | Cat#444203 |
| Critical commercial assays | ||
| Neural Tissue Dissociation Kit (P) | Miltenyi Biotec | Cat#130-092-628 |
| Click-iT™EdU Alexa Fluor™ 594 Imaging Kit | Invitrogen | Cat#C10339 |
| E.Z.N.A.® HP Total RNA Kit | OMEGA bio-tek | Cat#R6812-01 |
| RNase-free DNase I Set | OMEGA bio-tek | Cat#E1091 |
| Experimental models: Organisms/strains | ||
| Mouse: B6129SF1/J (14 days old, male or female) | The Jackson Laboratory | Cat#101043 |
| Software and algorithms | ||
| IncuCyte® ZOOM Software | Essen Bioscience | N/A |
| Fiji Image J | Schindelin et al., 2012 | https://imagej.net/software/fiji/ |
| Angiogenesis Analyzer for Image J | Carpentier, 2012 | https://imagej.nih.gov/ij/macros/toolsets/Angiogenesis%20Analyzer.txt |
| Other | ||
| 70 μM pore size strainer | VWR | Cat#76327-100 |
| LS columns | Miltenyi Biotec | Cat#130-042-401 |
| LD columns | Miltenyi Biotec | Cat#130-042-901 |
| MACS® MultiStand | Miltenyi Biotec | Cat#130-042-303 |
| QuadroMACS™ separator | Miltenyi Biotec | Cat#130-090-976 |
| IncuCyte® ImageLock Plates | Essen Bioscience | Cat#4379 |
| IncuCyte® 96-well WoundMaker Tool | Essen Bioscience | Cat#4563 |
| German glass coverslips, 12 mm round, #1 thickness | Mandel | Cat#NEU-GG-12-1.5-OZ |
| Corning® cryogenic vials, internal thread | Millipore sigma | Cat#CLS431386 |
| Mr. Frosty™ Freezing Container | Thermo Fisher Scientific | Cat#5100-0001 |
| Superfrost Plus Microscope Slides | Fisher Scientific | Cat#12-550-15 |
| 96-Well Tissue Culture Plate, Non-treated, Sterilized, Non-Pyrogenic | VWR | Cat#10861-562 |
| IncuCyte™ Zoom® Apparatus | Essen Bioscience | N/A |
| Nikon Eclipse TE2000-E inverted microscope | Nikon | N/A |
| Zeiss Axio Imager M2 microscope | Zeiss | N/A |
| Axiocam 506 mono | Zeiss | N/A |
| ApoTome.2 module | Zeiss | N/A |
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