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Organoid formation assay for survival assessment after PDO infection
(A) Representative bright-field images of non-infected (mock) or OAd-infected PDOs (0.02 MOI for 5 days) and re-seeded at a 1:2 split ratio. Examples of organoids (round-shaped, > 20 μm diameter) formed 24 h after reseeding are indicated with arrowheads.
(B) Quantification of organoids formed 24 h after re-seeding. Data are represented as mean ± SEM (n ≥ 5).
