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Basal medium, with (F12+++B) or without (F12+++) BSA
| Reagent | Final concentration | Amount |
|---|---|---|
| Advanced DMEM/F-12 | N/A | 500 mL |
| HEPES (1 M) | 10 mM | 5 mL |
| Pen/Strep | 1% | 5 mL |
| GlutaMAX | 2 mM (1%) | 5 mL |
| BSA 30% (for F12+++B) | 0.1% | 1.72 mL |
| Total | n/a | 516.72 mL |
Once prepared, store at 4°C
