REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-V5 antibody (1:1000 dilution)	Invitrogen	R960-25
Anti-beta Actin (1:1000 dilution)	Abcam	ab8227
Anti-mouse IgG IRDye 680RD (1:10000 dilution)	LI-COR Biosciences	925-68070
Anti-rabbit IgG IRDye 800RD (1:10000 dilution)	LI-COR Biosciences	926-32211
Anti-biotin Streptavidin AlexaFluor680 (1:1000 dilution)	Thermo Fisher Scientific	S32358
Recombinant DNA
pAAV-FLEx-ER-TurboID plasmid	Addgene	160857
Chemicals, peptides, and recombinant proteins
Biotin	Sigma-Aldrich	B4501-1G
DTT	Sigma-Aldrich	D0632-1G
Sodium azide	Sigma-Aldrich	S2002-25G
Sodium chloride	Sigma-Aldrich	S9888-500G
Sodium deoxycholate	Sigma-Aldrich	D6750-100G
Sodium dodecyl sulfate	Thermo Fisher Scientific	BP166-500
Sodium carbonate	Thermo Fisher Scientific	S263-500
Sodium bicarbonate	Thermo Fisher Scientific	187508
Sodium ascorbate	Thermo Fisher Scientific	A0539500G
Dimethyl sulfoxide	Sigma-Aldrich	D8418-100ML
Potassium chloride	Sigma-Aldrich	P3911-500G
EDTA	Sigma-Aldrich	E9884-100G
Tris-HCl	Sigma-Aldrich	1185-53-1
Acetonitrile	Thermo Fisher Scientific	A998-4
HALT protease inhibitor	Thermo Fisher Scientific	78429
NP-40 alternative	Merck Millipore	492016-100ML
Chloroform	Thermo Fisher Scientific	C607-4
Ponceau S solution	Sigma-Aldrich	P7170-1L
NuPAGE MOPS SDS Running Buffer (20×)	Invitrogen	NP0001-02
NuPAGE 4-12% Bis-Tris Protein Gels, 1.0 mm, 15-well	Thermo Fisher Scientific	NP0323BOX
Odyssey blocking buffer	LI-COR Biosciences	927-50000
Seeblue plus 2 protein ladder	Thermo Fisher Scientific	LC5925
NuPAGE™ LDS Sample Buffer (4×)	Thermo Fisher Scientific	NP0008
Urea	Sigma-Aldrich	31K0038
Acetone	Thermo Fisher Scientific	A184
Formic acid	Thermo Fisher Scientific	A117-50
Phosphoric acid	Sigma-Aldrich	345245-100ML
Trolox	Sigma-Aldrich	648471-500MG
0.9% saline solution	Teknova	S5825
TEAB solution (1M)	Sigma-Aldrich	T7408-100ML
Kolliphor EL	Sigma-Aldrich	C5135-500G
Trypsin	Promega	V5113
Iodoacetamide	Sigma-Aldrich	A3221
Critical commercial assays
SilverQuest™ Silver Staining Kit	Thermo Fisher Scientific	LC6070
Trans-blot turbo RTA transfer kit, nitrocellulose	Bio-Rad Laboratories	1704271
Experimental models: Organisms/strains
C57BL/6J (M. musculus)	The Jackson Laboratory	000664
Albumin-Cre mice	The Jackson Laboratory	003574
Bacterial and virus strains
One Shot™ TOP10 Chemically Competent E. coli	Invitrogen	C404010
Oligonucleotides
pENN forward: gcctctgctaaccatgttcatg	Integrated DNA Technologies	N/A
pENN reverse: gcagcgtatccacatag	Integrated DNA Technologies	N/A
Deposited data
Proteomic data	This study	ProteomeXchange: PXD021602
Processed proteomic datasets	Wei et al. (2021)	N/A
Other
3 kDa Molecular Weight Cutoff Filter	Amicon	UFC9003
EcoRI	New England Biolabs	R0101S
HindIII	New England Biolabs	R0104S
SmaI	New England Biolabs	R0141S
S-Trap™ micro columns	ProtiFi	C02-micro-40
Exel International Insulin Syringes	Thermo Fisher Scientific	14-841-32
lithium heparin tube	BD	365985
PrecisionGlide Needle 21G	BD	305129
Exel International Insulin Syringes 29G	Exel International	14-841-32
Tailveiner Restrainer for Mice	BRAINTREE SCIENTIFIC, INC.	TV-150 STD
Streptavidin magnetic beads	Thermo Fisher Scientific	65601
Bulk tubes	Thermo Fisher Scientific	15340162
Metal Bulk Beads	Thermo Fisher Scientific	15340158
Benchmark BeadBlaster Homogenizer	Thermo Fisher Scientific	15-340-163
Orbitrap Fusion Tribrid Mass Spectrometer	Thermo Fisher Scientific	N/A
NanoDrop Spectrophotometer	Thermo Fisher Scientific	ND-ONE-W
Dionex UltiMate 3000 RPLCnano system	Thermo Fisher Scientific	N/A
Acclaim PepMap 100 C18, 5-μm particles	Thermo Fisher Scientific	164213
Acclaim™ PepMap™ 100 C18 HPLC Columns (75 μm, packed with 2-μm, 100-Å)	Thermo Fisher Scientific	164942