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TFIIA mutants defective for TAF40 and TBP interaction cannot support cell viability. The growth of strains containing the indicated Toa2 derivative (or vector) was analyzed by spotting approximately 104 cells onto plates deficient for tryptophan and containing (+) or lacking (−) 5-FOA. When wild-type Toa2 (carried on a URA3 plasmid) is shuffled out on 5-FOA, cell viability is dependent on Toa2 functions carried on the TRP1 plasmid. WT, wild type.
