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Representative MS2 spectra and MS2 fragment ion coverage maps with matched b and y ions of the 5+ charge state of four Aβ proteoforms: (A) Aβ1–42 from 903.663 m/z precursor with 68.3% sequence coverage (43 PrSMs). (B) Aβ1–40 from 866.4374 m/z precursor with 48.7% sequence coverage (6 PrSMs). (C) Aβ2–42 from 880.6570 m/z precursor with 40.0% sequence coverage (3 PrSMs). (D) Aβ4–42 from 840.6410 m/z precursor with 44.7% sequence coverage (4 PrSMs).
