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. 2021 Dec 1;12:756131. doi: 10.3389/fphar.2021.756131

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Copyright © 2021 Gong, Zheng, Lyu, Dong, Tan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AGK2 decreases fibrogenic gene expression in TGF-β1-induced lung fibroblasts activation. (A,C) Protein and mRNA expression of Sirt2, Fibronectin and α-SMA proteins by Western blot and real-time RT-PCR in MRC-5 cells treated with 2 ng/ml TGF-β1 for 24 h and then added 10 μM AGK2 or DMSO for another 24 h in the presence of TGF-β1. GAPDH was used as loading control. (B) Densitometric analyses of the Western blot in (A). (D) Fibronectin and α-SMA was strongly expressed in response to TGF-β1, and AGK2 decreased the expression by immunofluorescence staining. Green means Fibronectin and α-SMA staining; Blue means DAPI. The bars indicated mean ± SD of three separate experiments. DMSO, dimethyl sulfoxide; **p <0.01, ***p <0.001, ****p <0.0001 compared to the TGF-β1+DMSO group.