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. 2021 Dec 1;12:756131. doi: 10.3389/fphar.2021.756131

FIGURE 3.

FIGURE 3

Silencing Sirt2 inhibits fibrogenic gene expression in TGF-β1-treated lung fibroblasts and IPF lung fibroblasts. (A,C) MRC-5 cells were transfected with NC siRNA or Sirt2 siRNA for 24 h, Sirt2 siRNA successfully downregulated Sirt2 protein and mRNA expression detected by Western blot and real-time RT-PCR. (B) Densitometric analyses of the Western blot in (A). (D,F) Protein and mRNA expression of Sirt2, Fibronectin, and α-SMA by Western blot and real-time RT-PCR in MRC-5 cells transfected with NC siRNA or Sirt2 siRNA for 24 h in the absence or presence of 2 ng/ml TGF-β1. (E) Densitometric analyses of the Western blot in (D). (G) Expression of Sirt2, Fibronectin and α-SMA by Western blot in IPF lung fibroblasts treated with NC siRNA or Sirt2 siRNA for 24 h. (H) Densitometric analyses of the Western blot in (G). GAPDH or β-actin was used as loading control. The bars indicated mean ± SD of three separate experiments. NC, negative control; siRNA, small interfering RNA. **p <0.01, ***p <0.001, ****p <0.0001.