Figure 2. Blockage of OMV‐induced E‐cadherin reduction in SNX10‐deficient cells is due to the inhibited nuclear localization of Snail/Slug proteins.

• A, B
  WT and SNX10 KO Caco‐2 cells were treated with or without OMVs (100 μg/ml) for 24 h. The fold changes in mRNA levels of SNAI1 (encoding Snail) (A) and SNAI2 (encoding Slug) (B) in Caco‐2 cells were determined by RT–qPCR. n = 6 independent experiments. Data are means ± SD. One‐way ANOVA followed by Bonferroni post hoc test was used for statistical analyses. ns, not significant.
• C
  WT and SNX10 KO Caco‐2 cells were treated with or without OMVs (100 μg/ml) for 24 h. Cytoplasmic and nuclear proteins were extracted for Snail and Slug determination by immunoblots.
• D–F
  WT and SNX10 KO Caco‐2 cells were treated with or without OMVs (100 μg/ml) for 24 h. Snail and Slug were assessed by immunofluorescence staining (D) and quantified as the percentage of Snail (E) or Slug (F)‐positive nuclei, respectively. Scale bar: 20 μm. n = 3 independent experiments, n = 18 fields analyzed. Data are means ± SD. One‐way ANOVA followed by Bonferroni post hoc test was used for statistical analyses. ***P < 0.001.
• G
  Caco‐2 cells were transfected with Ad‐vector or Ad‐SNX10, followed by treatment with or without 100 μg/ml OMVs for 24 h. Proteins from nucleus, cytoplasm, and total cell lysates were subjected to immunoblots with the indicated antibodies.
• H
  WT and SNX10 KO Caco‐2 cells were transfected with Ad‐vector or Ad‐SNX10, followed by treatment with or without 100 μg/ml OMVs for 24 h. Proteins from nucleus, cytoplasm, and total cell lysates were subjected to immunoblots with the indicated antibodies.

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