Figure 7. SNX10 deficiency protects against IL‐10 deficiency‐induced chronic colitis and maintains the intestinal barrier function in mice.

SNX10 conditional knockout (SNX10 cKO) mice with or without IL‐10 KO background and littermate wild‐type (WT) mice with or without IL‐10 KO background were compared (n = 6 animals, each group).

• A
  Body weight of mice was measured every 2 weeks from the 4^th week to 16^th week. Data are means ± SD. One‐way ANOVA followed by Bonferroni post hoc test was used for statistical analyses. **P < 0.01 and ***P < 0.001 versus WT group; ^## P < 0.01 and ^### P < 0.001 versus IL‐10 KO group.
• B, C
  Length of colons from WT, SNX10 cKO, IL‐10 KO, and SNX10 cKO IL‐10 KO mice was measured (B) and analyzed (C) at the 16^th week.
• D
  Clinical score was evaluated every 2 weeks from the 4^th week to the 16^th week. Data are means ± SD. One‐way ANOVA followed by Bonferroni post hoc test was used for statistical analyses. ***P < 0.001 versus WT group; ^### P < 0.001 versus IL‐10 KO group.
• E, F
  Representative H&E images of colon tissues of WT, SNX10 cKO, IL‐10 KO, and SNX10 cKO IL‐10 KO mice at the 16^th week were shown (E) and analyzed (F). Scale bar: 100 μm.
• G
  The content of TNF‐α, IL‐6, IL‐12/IL‐23 p40, and IL‐17A in the serum of WT, SNX10 cKO, IL‐10 KO, and SNX10 cKO IL‐10 KO mice at the 16^th week were measured by ELISA.
• H
  The relative mRNA levels of Tnf, Il6, Il23a, and Il17a in colon epithelial tissues of WT, SNX10 cKO, IL‐10 KO, and SNX10 cKO IL‐10 KO mice at the 16^th week were measured.
• I
  Representative immunohistochemistry images of E‐cadherin, Snail and Slug in colon tissues from WT, SNX10 cKO, IL‐10 KO, and SNX10 cKO IL‐10 KO mice at the 16^th week were shown. Scale bar: 100 μm.
• J
  Protein expression of E‐cadherin, p‐Lyn, and Lyn in colon epithelial tissues was determined by immunoblots.
• K
  The relative mRNA levels of Cdh1 (encoding E‐cadherin) in colon epithelial tissues were measured.
• L
  The gut permeability in vivo was evaluated using FITC‐dextran.

Data information: In (C and F–L), data are means ± SD. One‐way ANOVA followed by Bonferroni post hoc test was used for statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.001.

Source data are available online for this figure.