Table 2.

Primer and probe sequences used in rRT-PCR assays for chicken astrovirus and avian nephritis virus.

Virus	Primer location	Primer sequences	Product size (base pairs)	Reference
Chicken astrovirus	ORF1b, ORF2	Forward: 5′-GCTGCTGCTGAAGATATACAG-3′	70	Smyth et al. (17)
		Reverse: 5′-CATCCCTCTACCAGATTTTCTGAAA-3′
		Probe: 5′-6FAM CAGAAGTCGGGCCC BHQ1-3′
Avian nephritis virus	3′-Untranslated region	Forward: 5′-GTAAACCACTGGTTGGCTGACT-3′	56
		Reverse: 5′-TACTCGCCGTGGCCTCG-3′
		Probe: 5′-6FAM CAGCAACTGACTTTC BHQ1-3′