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. 2021 Dec 1;11:788875. doi: 10.3389/fonc.2021.788875

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Copyright © 2021 Smith, Cao, Chen, Mahmood, Phillips, Sutton and Cato

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of murine gastric cancer cells in vitro. (A) mRNA expression of CCK-BR is increased greater than 60-fold in YTN and NCC gastric cancer cells compared to normal mouse tissue. (B) mRNA expression by qRT-PCR of PD-L1 is markedly increased in gastric cancer YTN and NCC cells compared to normal tissues. (C) Exogenous gastrin stimulates growth of murine NCC gastric cancer cells in vitro (P = 0.004). **Significantly different from control. (D) Gastrin peptide expression is detected in NCC gastric cancer cells. (E) Gastrin peptide expression is detected in YTN gastric cancer cells. (F) Control cells stained with the secondary antibody only show no evidence of nonspecific immunoreactivity. Scale bar 200 μm.