Figure 2.

Uptake kinetics of the corona-coated nanoparticles isolated from full human plasma. Brain (a and e), lung (b and f), liver (c and g), and kidney endothelium (d and h) were exposed to 50 μg mL^–1 of 100 nm (a–d) or 30 μg mL^–1 of 200 nm (e–h) corona-coated SiO₂, SiO₂–NH₂, or SiO₂–COOH in serum-free medium, isolated from full human plasma as described in the Materials and Methods section. The results show the median cell fluorescence intensity of two replicate samples, together with a line that passes through their average. A linear regression two-tailed Student’s t-test was applied to compare the uptake of the different corona-coated nanoparticles. Statistically significant differences up to a 7 h uptake (indicated by an asterisk) are observed for all cell types on 100 nm nanoparticles for SiO₂–COOH as compared to the other functionalizations and on 200 nm nanoparticles for SiO₂–NH₂. p ≤ 0.05 was considered significant.