| Component | Per reaction (μl) | Per 120 reactions (μl) |
Final concentration in 13.5-μl reaction |
|---|---|---|---|
| 10× CutSmart buffer | 0.3 | 36 | 1× |
| T4 ligase (2,000 U/μl) | 0.5 | 60 | ~66.7 U/μl |
| 100 mM ATP | 0.1 | 12 | ~0.67 mM |
| H2O | 0.6 | 72 | — |
| Total | 1.5 | 180 | — |
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