| Order | Temperature (°C) | Duration (min) | Purpose |
|---|---|---|---|
| 1 | 98 | 5 | Denature dsDNA |
| 2 | 60 | 20 | Convert unmethylated cytosine to uracil |
| 3 | 98 | 5 | Denature dsDNA |
| 4 | 60 | 20 | Convert unmethylated cytosine to uracil |
| 5 | 20 | Hold | — |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.