| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 98 °C, 45 s | — | — |
| 6 | 98 °C, 20 s | 58 °C, 30 s | 72 °C, 1 min |
| 11 | 98 °C, 20 s | 65 °C, 30 s | 72 °C, 1 min |
| 1 | — | — | 72 °C, 5 min |
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