| Component | Per sample (μl) |
Per 120 samples (μl) |
Final concentration in 25-μl reaction |
|---|---|---|---|
| 2× KAPA HiFi HotStart ReadyMix | 12.5 | 1,500 | 1× |
| 10 μM PCR primer | 0.25 | 30 | 100 nM |
| H2O | 2.25 | 270 | — |
| Total | 15 | 1,800 | — |
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